Gene Targeting: ES Cell Electroporation

Gene targeting: es cell electroporation

Service Price
Oregon
Universities
Non-Oregon
Universities
     
Electroporation
$3140
$4710

 

Service Request

  1. Fill out a service request form and send to hgem@uoneuro.uoregon.edu.
  2. Please send a copy of your IACUC approved protocol and your IBC registration information.
  3. Provide a minimum of 50 μg of linearized DNA construct.

 

Service Description

  1. Electroporate the DNA into ES cells
  2. Perform drug-selection
  3. Pick drug-resistant colonies
  4. Freeze master plates and replicas for genotyping
  5. Expand and freeze up to six ES cell positive clones

 

Service Guaranties

A minimum of 2 x 96 colonies will be picked. However the TMF cannot guarantee the successful isolation of targeted ES cell clones.