Blastocyst Injection

Blastocyst Injection

Service Price
Oregon 
Universities
Non-Oregon
Universities
     
Blastocyst injection into C57Bl/6J
$2800
$5330
     
Breeding of Chimeras
(two litters max.)
$485
$730
Pathogen testing
(out-side clones)
$250
$250

 

Service Request

  1. Fill out a service request form and send to hgem@uoneuro.uoregon.edu.
  2. Please send a copy of your IACUC approved protocol and your IBC (Institutional Biosafety Committee) registration information.

 

Service Description

  1. Expand 2 ES cell clones on MEF feeders
  2. Purchase egg donors and recipient females
  3. Mate Superovulated donors with stud males
  4. Mate pseudopregnant foster mothers with vasectomized males
  5. Harvest morula or blastocyst from superovulated donors
  6. Inject ES cells into blastocoels
  7. Ovarian or uterian transfer of successfully injected blastocysts
  8. Wean and ear tag of resulting offspring
  9. Transfer of chimeras to the client

 

Service Guaranties

2 independent ES cell clones per project will be injected into a minimum of 50 blastocysts each.