Paraffin section

Wax Sections (Paraffin section)

Paraffin sectioning is the sectioning of tissue in which the water has been replaced with wax. An automated processor is used to replace the water in the tissue with wax. After processing, the tissue is embedded in paraffin and sectioned. We cut at 7 µm and collect the sections on a glass slides of the researcher's choice. Samples should be submitted in fixative. We will process the samples, embed them in paraffin, and section them There is also the option to have the sections stained before being sent back to the researcher for analysis.

For diagnostic purposes, we cut and mount 2 sections/slide. See image 2. For research purposes, we developed the technique to put up to 250 sections on a slides.

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There is also a one page form that the researcher needs to fill out regarding how the researcher would like their samples to be sectioned, what kind of slides they would like to be used, any special instructions regarding the sample, account number and contact information.

Routinely sectioned tissure:

  • Adult zebrafish
  • Zebrafish fin
  • Zebrafish gut
  • Zebrafish gonad
  • Embryonic zebrafish
  • Adult stickleback fish
  • Embryonic stickleback fish
  • Stickleback fish lower jaw
  • Stickleback fish gonad

Plastic Section (JB-4)

Plastic sectioning is special ultra-thin sectioning by embedding the samples in JB-4 plastic and sectioning at 3 microns. The sections are then collected on a glass slide. Our lab had refined the technique to collect all the sections serially on a slide in neatly arranged rows. With plastic sectioning, we can make very high quality section, ultra-thin without compression artifact. Due the technical difficulties inherent to this type of sectioning we can only cut limited quantities of small samples. Samples should be submitted to us in fixative. We will process them in JB-4 plastic, embed and section them.