The Histology and Genetic Modification Core Facility (HGeM) began in 2004 as the Transgenic Mouse Facility at the University of Oregon. This core facility was established by Dr. Clifford Kentros, Associate Professor of Psychology, and current Director Dr. Ute Hostick. Initial training was provided by the Transgenic Facility at Oregon Health and Science University (OHSU) under the leadership of Manfred Baetscher. The HGeM facility has since produced over one hundred unique transgenic mouse lines. Since 2007, we have offered additional services in gene targeting and blastocyst injections. The original transgenic core merged in 2013 with histology to form the Histology and Genetic Modification Core Facility (HGeM).
Our staff are continually improving techniques and services through formal training and partnership with other facilities. Examples include training at the The Jackson Laboratories in cryopreservation and recovery of cryopreserved material and a hands-on course in the Tetraploid Embryo Complementation Assay at the TCP at Mount Sinai Hospital, organized by Andras Nagy and Kristina Vintersten. In 2011, through a collaboration with the Zong lab, we worked with Linda Madisen at the Allen Institute for Brain Science in Seattle, WA to receive advanced training to refine ES cell techniques.
HGeM staff also maintain connections with active research through meetings and conferences, including regular attendance at International Society for Transgenic Technologies (ISTT) events, where we have been a member since 2007, the TT2008 meeting in Toronto, Ontario, Canada, the 2008 "Advances in Transgenic Animal Research" conference in Houston (TX, USA), at the MD Anderson Cencer Center, organized by Jan Parker-Thornburg in collaboration with ISTT as well as the TT2016 meeting in Prague, Czech Republic.
Adeno-associated viruses (AAVs) are commonly used in a range of neuroscience and cell biology experiments to express transgenes including fluorescent markers of optogenetic effectors. In order to offer additional services for creation, production, purification and titration of AAVs, we cross-trained with the Kentros lab at the Kavli Institute in Trondheim, Norway in the fall of 2019.