Services

 

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Services

 

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Cryosectioning

Cryo-sectioning is a technique for cutting frozen tissue samples which are then collected on glass microscope slides.

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Pronuclear Injection

Pronuclear injection is a very common and well established method used to create transgenic mice. This method is usually used for "gain-of-function" experiments.

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Custom AAV Production 

The core provides custom cloning and production of AAVs that are not yet commercially available. Our protocol produces high titer yields of serotypes 2.1 and 8.

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Paraffin Sectioning

Paraffin sectioning is the sectioning of tissue in which the water has been replaced with wax.

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Blast Injection

In order to study the "loss-of-function" of any given gene it is necessary to replace the endogenous gene with a modified construct. 

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Colony Management

Breeding colony management services covers day-to-day management and recordkeeping for your colony. 

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RNA In situ Hybridization

The core offers non-radioactive, RNA in situ hybridization (ISH) on frozen tissue sections primarily from rodent experimental models to determine gene expression patterns.

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CRISPR

CRISPR is a powerful gene editing tool, especially for the rapid introduction of point mutations and indels. We offer both pronuclear and ES cell-based approaches. 

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PCR Analysis

HGeM provides PCR-based genotyping of rodent lines for maintaining and determining trait zygosity. Custom assay development is also available.

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Immunohistochemistry

The core provides a full range of services including consultation, collection of animal tissue specimens, preparation of frozen sections, and documentation and quantification of expression patterns by microscopy. 

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Custom Cloning - Recombineering

Recombineering (recombination-mediated genetic engineering) is a novel and powerful method which enables fast and efficient construction of vectors to generate complex gene modifications in the mouse genome.

 

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Rederivation, Cryopreservation, IVF

Rederivation and Cryopreservation of mouse germplasm are essential tools for anyone working with mouse mutant strains. In vitro fertilization (IVF) is performed to recover a mouse strain from frozen sperm. 

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Staining

The standard histological stain is Hematoxilin and Eosin (H& E). This type staining is often use for quick analysis of tissue.

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Gene Targeting

In order to modify endogenous genes, it is necessary to perform gene targeting. A vector containing a modified copy of the gene of interest is electroporated into mouse embryonic stem (ES) cells.

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Image Acquisition

We offer high resolution, full-slice acquisition of epifluorescent and brightfield images. These images are suitable for anatomical analysis of gene expression patterns and viral infection.