Cryosectioning is a technique for cutting frozen tissue samples which are then collected on glass microscope slides. The tissue is rapidly frozen by floating the sample above liquid nitrogen. Samples are cut at temperatures ranging between -24o C to -28o C. The thickness we cut at depends on the researchers needs, from 16µm to 30µm.
Paraffin sectioning is the sectioning of tissue in which the water has been replaced with wax. An automated processor is used to replace the water in the tissue with wax. After processing, the tissue is embedded in paraffin and sectioned. We cut at 7 µm and collect the sections on a glass slides of the researcher's choice.
The standard histological stain is Hematoxilin and Eosin (H&E) The hemalum part of the stain dye the nuclei blue and the Eosin Y component stain eosinophilic structure in various shade of red, pink and orange. Researchers can derive abundant information from this stain. This type staining is often use for quick analysis of tissue.
Non-radioactive RNA in situ hybridization (ISH) on frozen tissue sections, primarily from rodent experimental models to determine gene expression patterns. The core provides a full range of services including consultation, collection of animal tissue specimens, preparation of frozen sections, probe labeling by in vitro transcription, conducting the ISH procedure, and documentation and quantification of expression patterns by microscopy.
Immunohistochemistry on fixed/frozen or floating tissue sections. The core has used the technique to characterize transgenic cells in the rodent brain with antibodies that recognize interneuron-specific markers parvalbumin and somatostatin. The core provides a full range of supporting services including consultation, collection of animal tissue specimens, cryosectioning, and image acquisition.
We offer high throughput, multi-slide acquisition of epi-fluorescent and brightfield signal using Leica's Aperio VERSA 200. The scanner is suitable for anatomical analysis of gene expression patterns and viral infection. It has 7 distinct fluorescent channels and standard magnifications up to 63X. HGeM also provides a full range of supporting services including preparation of tissue for scanning, scan set-up, and storage using the eSlide manager program.